pycA [2017-02-27 15:02:04]

pyruvate carboxylase

locus: BSU14860

pI: 5.00

mw: 127.00 kDa

protein length: 1148 aa

gene length: 3444 bp

function: replenishment of the oxaloacetate pool

product: pyruvate carboxylase

essential: no

synonyms: ylaP

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.4|TCA cycle]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

This gene is a member of the following [SW|regulons]

• [SW|stringent response], [SW|TnrA regulon]

Gene

Coordinates on the chromosome (coding sequence): 1,554,185 -> 1,557,631

The protein

[SW|Cofactors]

• biotin [Pubmed|24816803]

Structure

• [PDB|3BG5] (''S. aureus'')

[SW|Localization]

• membrane associated [Pubmed|18763711]
• cytoplasm (homogeneously distributed throughout the cell) [Pubmed|24825009]

Additional information

• PycA binds to StrepTactin, and may be co-purified when purifying Strep-tagged proteins by [SW|SPINE].
• PycA is subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]

Expression and Regulation: [[this]]

Biological materials

Mutant

• MGNA-A544 (ylaP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/544 NBRP B. subtilis, Japan]
• GP793 (cat), available in [SW|Jörg Stülke]'s lab

Expression vector

• pGP1289 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Stülke] lab

References

Reviews

• 18613815,12769720,10229653,9597748,7780827

Original publications

• 18763711,20081037,24825009,25755103,24816803